ABSTRACT
Background: The incision in the oral cavity is also taken for gingivectomy. Fig leaves (Ficus carica Linn) extract contains compounds of flavonoid, terpenoids and tannins and has anti-inflammatory and antioxidant activities. The important markers of wound healing were fibroblasts, macrophages and collagen density. Aim and Objectives: To investigate the topical application of fig leaves extracts on Wistar rat wounds on the number of macrophages, fibroblasts and collagen density after treatment for three and seven days. Material and Methods: This research was performed on 24 rats by making incision wounds on the backs of Wistar rats and divided into control and treatment groups. The control groups were left untreated and the treatment groups were given fig leaves extract gel on the incision wound once every day during thee and seven days, and then the animals were sacrificed. Wound tissue was removed and fixed in 10% formalin solution for histopathological test. Then it was embedded in paraffin, and stained wit Hematoxylin–Eosin to observe fibroblasts and acrophages. The collagen density was observed by Masson's Trichome staining. Statistical analyses of fibroblasts and macrophages were using One-way Anova and Tukeys HSD. Collagen density was analyzed by using Kruskall-Wallis and Mann-Whitney test. Results:There were significant differences amongthe groups (p<0.005) on the number of fibroblasts, macrophages and collagen density after treatment for three and seven days. Conclusion: Application of fig leaves extract on Wistar rat wounds could increase the number of fibroblasts and macrophages but not collagen density in the wound healing process.
ABSTRACT
Background: Lactoferrin possesses the ability to promote migration and proliferation of fibroblasts which represents one potential reason for the use of lactoferrin to accelerate the healing process in gingival wounds. Aim and Objectives:To analyze the role of lactoferrin in Fibroblast Growth Factor 2 (FGF-2) and Vascular Endothelial Growth Factor (VEGF) expression in the healing process of gingival wounds. Material and Methods: Twenty eight male Wistar rats were divided into four treatment groups. All rats were incised using a full thickness method on the gingival anterior region of the mandible to which a single application of lactoferrin concentration 10 μg/ml, 20 μg/ml, 40 μg/ml and phosphate buffer saline (control) was made. On the third day post-application, the rats were sacrificed to enable removal of the gingival tissue on the mandible. The role of lactoferrin was evaluated based on FGF-2 and VEGF expression identified through immunohistochemical staining. Results: In general, the group administered ith lactoferrin showed higher FGF-2 and VEGF expression compared to that of the control group (p=0.000). The group treated with 40 μg/ml lactoferrin concentration showed a higher FGF-2 and VEGF expression than the groups treated with 10 μg/ml and 20 μg/ml (p=0.000) lactoferrin concentration. Conclusion: Lactoferrin concentration of 40 μg/ml can accelerate the healing process of gingival wounds by increasing FGF-2 and VEGF expression.